Using Box Counting Techniques for Measuring Shape of Colonies of Filamentous Micro-organisms

		/vol02/j_soddel/	© Copyright 1995
		Volume 02	Received: Accepted:	---- ----

Using Box Counting Techniques for Measuring Shape of Colonies of Filamentous Micro-organisms Jacques Soddell and Robert Seviour
Abstract
	Two box counting programs were assessed for their suitability for describing changes in the complex shape of colonies of filamentous bacteria in terms of fractal geometry. Despite using the same images, the two programs did not produce the same values for the fractal dimensions (D) calculated. One of the programs offered choice of box sizes as well as different starting positions for the box-counting grid, which resulted in better correlation between increases in complexity and higher fractal dimension. This flexibility was important because of the sensitivity to choice of box size found with known fractals which were also tested. D values obtained for colonies of Nocardia asteroides, Nocardia otitidiscaviarum and Rhodococcus erythropolis confirmed that fractal geometry is valuable for describing differences between colonies of these organisms.

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